Heterochromatin protein 1 alpha (HP1α) undergoes a monomer to dimer transition that opens and compacts live cell genome architecture

J Lou; Q Deng; X Zhang; CC Bell; AB Das; NG Bediaga; CO Zlatic; TM Johanson; RS Allan; MDW Griffin; PN Paradkar; KF Harvey; MA Dawson; E Hinde

Journal article

Heterochromatin protein 1 alpha (HP1α) undergoes a monomer to dimer transition that opens and compacts live cell genome architecture

J Lou, Q Deng, X Zhang, CC Bell, AB Das, NG Bediaga, CO Zlatic, TM Johanson, RS Allan, MDW Griffin, PN Paradkar, KF Harvey, MA Dawson, E Hinde

Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2024

DOI: 10.1093/nar/gkae720

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Abstract

Our understanding of heterochromatin nanostructure and its capacity to mediate gene silencing in a living cell has been prevented by the diffraction limit of optical microscopy. Thus, here to overcome this technical hurdle, and directly measure the nucleosome arrangement that underpins this dense chromatin state, we coupled fluorescence lifetime imaging microscopy (FLIM) of Förster resonance energy transfer (FRET) between histones core to the nucleosome, with molecular editing of heterochromatin protein 1 alpha (HP1α). Intriguingly, this super-resolved readout of nanoscale chromatin structure, alongside fluorescence fluctuation spectroscopy (FFS) and FLIM-FRET analysis of HP1α protein-protei..

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University of Melbourne Researchers

Jieqiong Lou Author

Andrew Das Author

Courtney Zlatic Author

Tim Johanson Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

J.L. was supported by an Excellence in Diversity Fellowship from the University of Melbourne and the Kaye Merlin Brutton Bequest; A.D. was supported by a John Gavin Fellowship from the Cancer Research Trust New Zealand; T.M.J. was supported by a National Health and Medical Research Council (NHMRC) Early Career Fellowship [1124081]; R.S.A. was supported by NHMRC Ideas Grants [1158531 and 2001131]; K.F.H. was supported by a NHMRC Investigator Grant [1194467]; M.A.D. was supported by a NHMRC Investigator Grant [1196749]; Australian Research Council (ARC) Discovery Project [DP220103927]; E.H was supported by a NHMRC Career Development Fellowship [1124762]; ARC Future Fellowship [FT200100401]; ARC Discovery Projects [DP180101387 and DP21010298]; Jacob Haimson Beverly Mecklenburg Lectureship.